绵羊 anti-人 kappa Light Chain (Free & Bound) Antibody
IP
Polyclonal
unconjugated
Quick Overview for 绵羊 anti-人 kappa Light Chain (Free & Bound) Antibody (ABIN458483)
抗原
kappa Light Chain
克隆类型
多克隆
应用范围
Immunoprecipitation (IP)
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抗原表位
- Free & Bound
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适用
- 人
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宿主
- 绵羊
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特异性
- Precipitating polyclonal Sheep antiSerum to free and bound Mouse Ig kappa light chain.
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交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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产品特性
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The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of mouse serum or plasma. To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immunodiffusion.
Clone: Polyclonal -
纯化方法
- Delipidated, heat inactivated lyophilized whole serum.
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过滤
- Heat-inactivated
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免疫原
- A pool of purified Bence Jones kappa proteins isolated from mouse urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
- In immunoelectrophoresis use 2 μL serum or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in 3 mm diameter center well and 2 μL serum samples (neat and serially diluted in 2 mm diameter peripheral wells. When carrying out analyses of Bence Jones proteins or free light chains in urine or serum using an immunodiffusion technique, special attention should be given to their faster rate of diffusion as compared to that of the antibody molecules in the antiserum. If they both start to diffuse into the gel in a double radial diffusion at the same time, an excess of the antigen may easily prevent visible precipitation. To prevent false negative results, the antiserum should be diffuse for about 2 hours before the antigen reservoir is filled.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles.Delipidated, heat inactivated lyophilized whole serum. No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles.
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缓冲液
- Delipidated, heat inactivated lyophilized whole serum. No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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储存条件
- RT,4 °C,-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- kappa Light Chain
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别名
- Ig kappa
抗原
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